1. Supply anatomy data for repair of anterior cranial fossa injuries using calvaria and galea aponeurotica vescularized flaps.
提供使用颅盖和盖利腱膜囊状皮瓣修复颅前窝损伤的解剖学数据。
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2. Methods The osteoblasts were isolated from human fetal calvaria.
方法体外分离培养人成骨细胞。
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3. Osteoblasts were delievered and cultured from the new-born Wistar rats calvaria by the digesting method.
采用消化法从初生大鼠的颅盖骨中分离、培养成骨细胞。
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4. Results The cells from rabbit calvaria were positive to ALP staining and could form calcium node in vitro.
结果从兔颅骨分离的细胞碱性磷酸酶染色阳性，在长期培养中能够形成钙化结节。
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5. Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
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6. Objective To supply anatomy data for repair of anterior cranial fossa injuries using calvaria and galea aponeurotica vescularized flaps.
目的为颅前窝组织缺损一次性修复重建提供研究资料。
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7. Criteria for inclusion of an article required content related the mechanism of calvaria abnormalities, and unrelated and repetitive articles were excluded.
纳入与颅盖畸形形成机制有关的内容，排除不相关及重复文章。
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8. Complications included pin loosening in 24, pin infection in 4, loss of the reduction in 4, penetration of inner plate of calvaria in 3 and pressure sores in 1 case.
9. Materils and method: Obtaining experimental cellular model by isolating and culturing in vitro of osteoblasts from SD rats' fetal calvaria . Differentiation stages were divided .
10. Conclusion Further research about craniosynostosis and the growth factors involved will contribute to understanding the mechanism of calvaria abnormalities and help treat problems.
11. Methods: the serum collected from experimental rats given soybean isoflavones extract was added to the culture system of osteoblast cells (ob) isolated from the calvaria of neonatal SD rats.
12. Methods: the serum collected from experimental rats given soybean isoflavones extract was added to the culture system of osteoblast cells (ob) isolated from the calvaria of neonatal SD rats.
