8. The cells combined human proinsulin gene has the ability of producing insulin with increase in glucose concentration in vitro.
所构建的重组人胰岛素原基因能指导靶细胞随葡萄糖浓度的变化调节胰岛素分泌。
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9. Secreted proinsulin amounted to about 2 mg per liter was found in the culture medium of yeast strain 63 transformed by plasmid YTI-15.
用表达载体yti - 15转化酵母63号株可得到每升约2毫克的人胰岛素原分泌产物。
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10. The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
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11. Receptor binding activity of the two mutants gives 66% and 54% of that of human proinsulin, respectively, while radio immuno activity drops to 22% and 9%.
12. Receptor binding activity of the two mutants gives 66% and 54% of that of human proinsulin, respectively, while radio immuno activity drops to 22% and 9%.