11. Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
方法利用B细胞杂交瘤技术,建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
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12. Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
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13. Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
14. To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体,鼠源性抗体可变区基因的克隆与测序是关键。
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15. Highly positive rate was obtained. The four hybridoma cells were separately cloned nine times in proper sequence and can continuously secrete high-titer McAbs.
16. The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
将纯化之球蛋白片段免疫 BALB/c小鼠后, 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1进行细胞融合。
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17. Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
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18. But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.
19. Since 1975 when the technology of hybridoma and monoclonal antibody was developed, many investigators have been paying close attention to antibody targeting therapy for patients with cancer.
自从1975年发现了单克隆抗体技术和不久后提出生物导弹设想以来,导向治疗受到许多科研工作者的关注。
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20. Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.
研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。
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21. Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.
研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。
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用作名词 (n.)
By this procedure, Milstein and Kohler developed a hybridoma that made a specific antibody. 米尔斯坦和科勒用此方法培养出一个杂种瘤,后来成为一种特效抗体。